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细胞系身份确认

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licheng 发表于 2015-12-23 13:44:47 | 显示全部楼层 |阅读模式
文献
http://www.nature.com/nature/journal/v520/n7547/full/nature14397.html
A resource for cell line authentication, annotation and quality control
提倡使用细胞系的研究都要通过STR或SNP确认细胞系的身份

http://www.nature.com/nmeth/journal/v11/n5/full/nmeth.2932.html
Cell-line authentication demystified

http://www.nature.com/nmeth/journal/v12/n6/full/nmeth.3403.html
Reproducibility: changing the policies and culture of cell line authentication
[url=http://journals.plos.org/plosone ... ournal.pone.0116218]
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116218[/url]
Human Biosample Authentication Using the High-Throughput, Cost-Effective SNPtraceTM System

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492511/
High-throughput SNP-based authentication of human cell lines

数据库:
http://iclac.org/databases/
The International Cell Line Authentication Committee

Hela细胞
GEO数据库中能找到很多于SNP芯片的Hela基因型(搜索“hela snp”),
http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM226875
结合芯片注释http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL2641
可以把A/B基因型转换为“SEQUENCE”
列中的SNP型,再与自己的测序数据SNP对比。

几十个SNP可以唯一确定人的基因型:
http://www.ncbi.nlm.nih.gov/pubmed/19937056
SNPs for a universal individual identification panel.

NCI60 cell lines
https://dtp.cancer.gov/discovery_development/nci-60/characterization.htm
Molecular Characterization of the NCI-60

http://www.ncbi.nlm.nih.gov/pubmed/20830292
A whole-genome SNP association study of NCI60 cell line panel indicates a role of Ca2+ signaling in selenium resistance
所用SNP数据Affymetrix 125K SNP array data:
https://wiki.nci.nih.gov/display/NCIDTPdata/Molecular+Target+Data

新闻:
http://www.promegaconnections.com/tag/cell-line-authentication/

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